Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli.

@article{Cress2016RapidGO,
  title={Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli.},
  author={Brady F Cress and J Andrew Jones and Daniel C. Kim and Quentin D Leitz and Jacob A Englaender and Shannon M. Collins and Robert J. Linhardt and Mattheos A G Koffas},
  journal={Nucleic acids research},
  year={2016},
  volume={44 9},
  pages={4472-85}
}
Robust gene circuit construction requires use of promoters exhibiting low crosstalk. Orthogonal promoters have been engineered utilizing an assortment of natural and synthetic transcription factors, but design of large orthogonal promoter-repressor sets is complicated, labor-intensive, and often results in unanticipated crosstalk. The specificity and ease of targeting the RNA-guided DNA-binding protein dCas9 to any 20 bp user-defined DNA sequence makes it a promising candidate for orthogonal… CONTINUE READING

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