Rapid gene splicing and multi-sited mutagenesis by one-step overlap extension polymerase chain reaction.

Abstract

Gene splicing and site-directed mutagenesis (SDM) are important to introduce desired sequences in target DNA. However, introducing mutations at multiple sites requires multiple steps of DNA manipulation, which is time-consuming and labor-intensive. Here, we present a rapid efficient gene splicing and multi-sited mutagenesis method that introduces mutations… (More)
DOI: 10.1016/j.ab.2012.06.027

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