Rapid fluorescence-based measurement of neutrophil migration in vitro.

@article{Frevert1998RapidFM,
  title={Rapid fluorescence-based measurement of neutrophil migration in vitro.},
  author={Charles W Frevert and Venus A. Wong and Richard B. Goodman and Riordan Goodwin and Thomas R. Martin},
  journal={Journal of immunological methods},
  year={1998},
  volume={213 1},
  pages={
          41-52
        }
}
We have standardized a new chemotaxis chamber that uses fluorescence as the cellular marker for the measurement of leukocyte migration in vitro in disposable 96-well microplates. This new fluorescence-based assay is a robust assay because filter pore size, cell density, filter composition, and filter thickness do not affect PMN migration towards interleukin-8 or the complement fragment, C5a. When compared to two separate chemotaxis assays in which the migrated cells are counted visually, the… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 70 CITATIONS

FILTER CITATIONS BY YEAR

1999
2018

CITATION STATISTICS

  • 3 Highly Influenced Citations

  • Averaged 2 Citations per year over the last 3 years

Similar Papers

Loading similar papers…