Rapid fluorescence-based measurement of neutrophil migration in vitro.

  title={Rapid fluorescence-based measurement of neutrophil migration in vitro.},
  author={Charles W Frevert and Venus A. Wong and Richard C. Goodman and Ray Goodwin and Thomas R. Martin},
  journal={Journal of immunological methods},
  volume={213 1},
We have standardized a new chemotaxis chamber that uses fluorescence as the cellular marker for the measurement of leukocyte migration in vitro in disposable 96-well microplates. This new fluorescence-based assay is a robust assay because filter pore size, cell density, filter composition, and filter thickness do not affect PMN migration towards interleukin-8 or the complement fragment, C5a. When compared to two separate chemotaxis assays in which the migrated cells are counted visually, the… CONTINUE READING

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