Rapid fluorescence assessment of intracellular pH as a viability indicator of Clavibacter michiganensis subsp. michiganensis.

@article{Chitarra2000RapidFA,
  title={Rapid fluorescence assessment of intracellular pH as a viability indicator of Clavibacter michiganensis subsp. michiganensis.},
  author={Luiz Gonzaga Chitarra and Peter Breeuwer and Ruud W van den Bulk and Tjakko Abee},
  journal={Journal of applied microbiology},
  year={2000},
  volume={88 5},
  pages={809-16}
}
The viability of Clavibacter michiganensis subsp. michiganensis (Cmm) was determined by measuring the intracellular pH (pHin) as a viability parameter. This was based on the observation that growth of Cmm was inhibited at pH 5.5 and below. Therefore, viable cells should maintain their pHin above this pH value. The pHin of Cmm was determined using the fluorescent probe 5(and 6-)-carboxyfluorescein succinimidyl ester (cFSE). The pHin of Cmm cells exposed to acid treatments was determined using… CONTINUE READING