Rapid extraction of genomic DNA from medically important yeasts and filamentous fungi by high-speed cell disruption.

  title={Rapid extraction of genomic DNA from medically important yeasts and filamentous fungi by high-speed cell disruption.},
  author={Florian M{\"u}ller and Katherine E. Werner and Miki Kasai and Andrea Francesconi and Stephen J. Chanock and Thomas J Walsh},
  journal={Journal of clinical microbiology},
  volume={36 6},
Current methods of DNA extraction from different fungal pathogens are often time-consuming and require the use of toxic chemicals. DNA isolation from some fungal organisms is difficult due to cell walls or capsules that are not readily susceptible to lysis. We therefore investigated a new and rapid DNA isolation method using high-speed cell disruption (HSCD) incorporating chaotropic reagents and lysing matrices in comparison to standard phenol-chloroform (PC) extraction protocols for isolation… CONTINUE READING


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