Rapid expression cloning of human immunoglobulin Fab fragments for the analysis of antigen specificity of B cell lymphomas and anti-idiotype lymphoma vaccination.

@article{Osterroth1999RapidEC,
  title={Rapid expression cloning of human immunoglobulin Fab fragments for the analysis of antigen specificity of B cell lymphomas and anti-idiotype lymphoma vaccination.},
  author={Frank Osterroth and Ozan Alkan and Andreas Mackensen and Andrea Lindemann and Paul Fisch and Arne Skerra and Hendrik Veelken},
  journal={Journal of immunological methods},
  year={1999},
  volume={229 1-2},
  pages={141-53}
}
An expression system for rapid and standardized production of human recombinant immunoglobulin Fab fragments in E. coli was developed. Functional folding of the Fab fragments was accomplished by the dicistronic expression vector pFab.gammakappa containing specialized leader sequences to direct the immunoglobulin heavy and light chains to the periplasmic bacterial space. A C-terminal hexahistidine tag of the Fd chain facilitated metal affinity chromatography and purification to homogeneity as… CONTINUE READING