Rapid exchange of subunits between free ribosomes in extracts of Escherichia coli.

@article{Subramanian1971RapidEO,
  title={Rapid exchange of subunits between free ribosomes in extracts of Escherichia coli.},
  author={Alap Raman Subramanian and Bernard D. Davis},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1971},
  volume={68 10},
  pages={
          2453-7
        }
}
The runoff of a mixture of "heavy" and "light" polysomes has been reported to yield hybrid ribosomes, of intermediate density. However, we found that the dissociation of free heavy ribosomes as they advance in a sucrose gradient causes a serious artefact in the demonstration of hybrid ribosomes. This artefact is eliminated by fixation with glutaraldehyde before sedimentation. Formation of hybrid ribosomes can then be demonstrated, but it is equally complete whether the heavy and light… CONTINUE READING

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