Rapid evolution of a protein in vitro by DNA shuffling

  title={Rapid evolution of a protein in vitro by DNA shuffling},
  author={Willem P. C. Stemmer},
DNA SHUFFLING is a method for in vitro homologous recombination of pools of selected mutant genes by random fragmentation and polymerase chain reaction (PCR) reassembly1. Computer simulations called genetic algorithms2–4have demonstrated the importance of iterative homologous recombination for sequence evolution. Oligonucleotide cassette mutagenesis5–11 and error-prone PCR12,13 are not combinatorial and thus are limited in searching sequence space1,14. We have tested mutagenic DNA shuffling for… CONTINUE READING
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