Rapid enumeration of respiratory active mycobacteria with fluorescent double staining.

@article{Ichijo2010RapidEO,
  title={Rapid enumeration of respiratory active mycobacteria with fluorescent double staining.},
  author={Tomoaki Ichijo and Yoko Izumi and Nobuyasu Yamaguchi and Masao Nasu},
  journal={Journal of microbiological methods},
  year={2010},
  volume={82 3},
  pages={
          327-9
        }
}
A new method for rapid enumeration of physiologically active mycobacteria was developed by acid-fast bacilli staining (Auramine O) following formazan reduction. Results can be obtained within 90 min by the optimized procedure, while more than one week is required for the widely used culture-dependent approach. 
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