Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA

@article{Gallagher2014RapidEA,
  title={Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA},
  author={Ryan R. Gallagher and Zhe Li and Aaron O Lewis and Farren J. Isaacs},
  journal={Nature Protocols},
  year={2014},
  volume={9},
  pages={2301-2316}
}
Multiplex automated genome engineering (MAGE) is a powerful technology for in vivo genome editing that uses synthetic single-stranded DNA (ssDNA) to introduce targeted modifications directly into the Escherichia coli chromosome. MAGE is a cyclical process that involves transformation of ssDNA (by electroporation) followed by outgrowth, during which bacteriophage homologous recombination proteins mediate annealing of ssDNAs to their genomic targets. By iteratively introducing libraries of… 

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