Rapid diagnosis of mycobacterial infections and quantitation of Mycobacterium tuberculosis load by two real-time calibrated PCR assays.

@article{Broccolo2003RapidDO,
  title={Rapid diagnosis of mycobacterial infections and quantitation of Mycobacterium tuberculosis load by two real-time calibrated PCR assays.},
  author={Francesco Broccolo and Paolo Scarpellini and Giuseppe Locatelli and Anna Zingale and Anna Maria Brambilla and Paola Cichero and Leonardo A. Sechi and Adriano Lazzarin and Paolo Lusso and Mauro Severo Malnati},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 10},
  pages={4565-72}
}
Sensitive and specific techniques to detect and identify Mycobacterium tuberculosis directly in clinical specimens are important for the diagnosis and management of patients with tuberculosis (TB). We developed two real-time PCR assays, based on the IS6110 multicopy element and on the senX3-regX3 intergenic region, which provide a rapid method for the diagnosis of mycobacterial infections. The sensitivity and specificity of both assays were established by using purified DNA from 71 clinical… CONTINUE READING