Rapid diagnosis of dengue viremia by reverse transcriptase-polymerase chain reaction using 3'-noncoding region universal primers.

@article{Sudiro1997RapidDO,
  title={Rapid diagnosis of dengue viremia by reverse transcriptase-polymerase chain reaction using 3'-noncoding region universal primers.},
  author={Tahjani Mirawati Sudiro and Hiroaki Ishiko and Sharone Green and David W. Vaughn and Ananda N Nisalak and Siripen Kalayanarooj and Alan Rothman and Boonyos Raengsakulrach and J. Mark Janus and Ichiro Kurane and Francis A. Ennis},
  journal={The American journal of tropical medicine and hygiene},
  year={1997},
  volume={56 4},
  pages={424-9}
}
A reverse transcriptase-polymerase chain reaction (RT-PCR) method was developed as a rapid diagnostic test of dengue viremia. To detect dengue viruses in serum or plasma specimens, a pair of universal primers was designed for use in the RT-PCR. Using these primers, the 3'-noncoding region of dengue virus types 1, 2, 3, and 4 could be amplified, but not those of other flaviviruses, such as West Nile virus, Japanese encephalitis virus, and yellow fever virus, or the alphavirus Sindbis virus. The… CONTINUE READING