Rapid diagnosis of azole-resistant aspergillosis by direct PCR using tissue specimens.

@article{Linden2010RapidDO,
  title={Rapid diagnosis of azole-resistant aspergillosis by direct PCR using tissue specimens.},
  author={Jan W.M. van der Linden and Eveline Snelders and Jan P A Arends and Simon M. G. J. Daenen and Willem J G Melchers and Paul E Verweij},
  journal={Journal of clinical microbiology},
  year={2010},
  volume={48 4},
  pages={1478-80}
}
We report the use of PCR techniques on a formalin-fixed and paraffin-embedded tissue specimen for direct detection of one dominant azole resistance mechanism in a case of disseminated invasive aspergillosis. Rapid detection of mutations associated with azole resistance directly in tissue significantly reduces diagnostic delay. 
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