Rapid development of sensitive, high-throughput, quantitative and highly selective mass spectrometric targeted immunoassays for clinically important proteins in human plasma and serum.

@article{Krastins2013RapidDO,
  title={Rapid development of sensitive, high-throughput, quantitative and highly selective mass spectrometric targeted immunoassays for clinically important proteins in human plasma and serum.},
  author={Bryan Krastins and Amol Prakash and David A. Sarracino and Dobrin Nedelkov and Eric E. Niederkofler and Urban A. Kiernan and Randall S Nelson and Maryann S Vogelsang and Gouri Vadali and Alejandra Garces and Jennifer N. Sutton and Scott M. Peterman and Gregory Byram and Bruno Darbouret and Jo{\"e}lle R. P{\'e}russe and Nabil G Seidah and Benoit Coulombe and Johan Gobom and Erik Portelius and Josef Pannee and K Blennow and Vathany Kulasingam and Lewis Couchman and C. V. D. Moniz and Mary F. Lopez},
  journal={Clinical biochemistry},
  year={2013},
  volume={46 6},
  pages={
          399-410
        }
}
OBJECTIVES The aim of this study was to develop high-throughput, quantitative and highly selective mass spectrometric, targeted immunoassays for clinically important proteins in human plasma or serum. DESIGN AND METHODS The described method coupled mass spectrometric immunoassay (MSIA), a previously developed technique for immunoenrichment on a monolithic microcolumn activated with an anti-protein antibody and fixed in a pipette tip, to selected reaction monitoring (SRM) detection and… CONTINUE READING
Highly Cited
This paper has 32 citations. REVIEW CITATIONS

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 20 extracted citations

Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences • 2016
View 4 Excerpts
Method Support
Highly Influenced

Plasma PCSK9 measurement by liquid chromatography-Tandem mass spectrometry and comparison with conventional ELISA.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences • 2017

Antibodies as means for selective mass spectrometry.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences • 2016
View 1 Excerpt