Rapid development of molecular markers by next-generation sequencing linked to a gene conferring phomopsis stem blight disease resistance for marker-assisted selection in lupin (Lupinus angustifolius L.) breeding

@article{Yang2012RapidDO,
  title={Rapid development of molecular markers by next-generation sequencing linked to a gene conferring phomopsis stem blight disease resistance for marker-assisted selection in lupin (Lupinus angustifolius L.) breeding},
  author={Huaan Yang and Ye Tao and Zequn Zheng and Di Shao and Zhenzhong Li and Mark W. Sweetingham and Bevan J. Buirchell and Chengdao Li},
  journal={Theoretical and Applied Genetics},
  year={2012},
  volume={126},
  pages={511-522}
}
Selection for phomopsis stem blight disease (PSB) resistance is one of the key objectives in lupin (Lupinus angustifolius L.) breeding programs. A cross was made between cultivar Tanjil (resistant to PSB) and Unicrop (susceptible). The progeny was advanced into F8 recombinant inbred lines (RILs). The RIL population was phenotyped for PSB disease resistance. Twenty plants from the RIL population representing disease resistance and susceptibility was subjected to next-generation sequencing (NGS… CONTINUE READING
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