Rapid development of microsatellite markers for the critically endangered Saiga (Saiga tatarica) using Illumina® Miseq next generation sequencing technology

Abstract

We developed 17 variable microsatellite markers for the threatened Saiga antelope. Using one Illumina Miseq lane resulted in 105,948 unique fragments containing a microsatellite motif. Eighty-one ordered primer pairs resulted in 26 analyzable fragments, of which 17 markers showed variability in at least one population from Kazakhstan. Number of alleles… (More)
DOI: 10.1007/s12686-013-0033-3

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