Rapid determination of the amino acid configuration of xenotetrapeptide.

@article{Kegler2014RapidDO,
  title={Rapid determination of the amino acid configuration of xenotetrapeptide.},
  author={C. Kegler and Friederike Inga Nollmann and Tilman Ahrendt and Florian Fleischhacker and Edna Bode and Helge B Bode},
  journal={Chembiochem : a European journal of chemical biology},
  year={2014},
  volume={15 6},
  pages={
          826-8
        }
}
An E. coli strain with deletions in five transaminases (ΔaspC ΔilvE ΔtyrB ΔavtA ΔybfQ) was constructed to be unable to degrade several amino acids. This strain was used as an expression host for the analysis of the amino acid configuration of nonribosomally synthesized peptides, including the novel peptide "xenotetrapeptide" from Xenorhabdus nematophila, by using a combination of labeling experiments and mass spectrometry. Additionally, the number of D-amino acids in the produced peptide was… CONTINUE READING
BETA
1
Twitter Mention