Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification.

@article{Chen2011RapidDO,
  title={Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification.},
  author={Siyi Chen and Fei Wang and John C Beaulieu and Rebecca E. Stein and Beilei Ge},
  journal={Applied and environmental microbiology},
  year={2011},
  volume={77 12},
  pages={4008-16}
}
Recent outbreaks linked to Salmonella-contaminated produce heightened the need to develop simple, rapid, and accurate detection methods, particularly those capable of determining cell viability. In this study, we examined a novel strategy for the rapid detection and quantification of viable salmonellae in produce by coupling a simple propidium monoazide sample treatment with loop-mediated isothermal amplification (PMA-LAMP). We first designed and optimized a LAMP assay targeting Salmonella… CONTINUE READING