Rapid detection of the Marek's disease viral genome in chicken feathers by loop-mediated isothermal amplification.

@article{Angamuthu2012RapidDO,
  title={Rapid detection of the Marek's disease viral genome in chicken feathers by loop-mediated isothermal amplification.},
  author={Raja Angamuthu and Subasty Baskaran and Dhinakar Raj Gopal and Jeyanthi Devarajan and Kumanan Kathaperumal},
  journal={Journal of clinical microbiology},
  year={2012},
  volume={50 3},
  pages={961-5}
}
A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did the LAMP primers have binding sites for the common avian DNA viruses (reticuloendotheliosis virus, chicken anemia virus, subgroup J of the avian leukosis virus). Additionally, the… CONTINUE READING