Rapid detection of mutagen induced micronucleated erythrocytes by flow cytometry

@article{Hutter2004RapidDO,
  title={Rapid detection of mutagen induced micronucleated erythrocytes by flow cytometry},
  author={K. Hutter and M. St{\"o}hr},
  journal={Histochemistry},
  year={2004},
  volume={75},
  pages={353-362}
}
SummaryThe micronucleus test is a cytogenetic method for the screening of mutagens and carcinogens which exhibit clastogenic mechanisms of action. After application of clastogenic agents chromosomal fragments or even whole chromsomes can remain as conspicuous structures (micronuclei) in a small fraction of anucleated polychromatic erythrocytes which can be visually scored using a microscope following staining with May-Grünwald-Giemsa solutions. These time-consuming, painstaking, and tedious… Expand
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TLDR
The feasibility of using MN-RET frequencies in blood from humans as an index of genetic damage in bone marrow opens a critical area of application that had not been practical previously. Expand
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TLDR
The micronucleus assay using cells other than bone marrow cells, for example using liver, skin, testis, and colon cells, has been developed and validated successfully and the automated evaluation systems introduced to the test system with great success are discussed here. Expand
The use of immunomagnetic separation of erythrocytes in the in vivo flow cytometer-based micronucleus assay.
TLDR
The flow cytometer-based micronucleus assay by use of a cytometer equipped with two lasers is refined, with immunomagnetic separation of the very youngest erythrocytes - which are transferrin-positive (Trf+Ret) - prior to analysis, and an almost pure (>98%) Trf+ Ret-population is obtained. Expand
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Differentiation of Aneugens and Clastogens in the In Vitro Micronucleus Test by Kinetochore Scoring Using Automated Image Analysis
TLDR
A novel method for automated MN plus kinetochore (k+) scoring by image analysis was developed based on the OECD TG 487 and demonstrates excellent specificity and sensitivity and the methodology is superior to manual microscopic analysis in terms of speed and throughput as well as the absence of human bias. Expand
Laser scanning cytometry for automation of the micronucleus assay.
TLDR
The advances to date in measuring MN in cell lines, buccal cells and erythrocytes, and the expansion of MN analysis by LSC to other models hold great promise for robust and exciting developments in MN assay automation as a high-content high-throughput analysis procedure. Expand
8 Monitoring Vivo Cytogenetic Damage In
Chromosomal alterations have been used as an important biological endpoint to study the mutagenic effects of ionizing radiation and chemicals. Some basic principles in radiobiology were based onExpand
In vivo erythrocyte micronucleus assay III. Validation and regulatory acceptance of automated scoring and the use of rat peripheral blood reticulocytes, with discussion of non-hematopoietic target cells and a single dose-level limit test.
TLDR
The working group discussed new aspects in the in vivo micronucleus test, including the regulatory acceptance of data derived from automated scoring, the use of flow cytometry, the suitability of rat peripheral blood reticulocytes to serve as the principal cell population for analysis, the establishment of in vivo MN assays in tissues other than bone marrow and blood, and the biological relevance of the single-dose-level test. Expand
The impact of air pollution on the levels of micronuclei measured by automated image analysis.
TLDR
This work is the first human biomonitoring study focused on the measurement of micronuclei by automated image analysis for assessing chromosomal damage as a result of environmental mutagen exposure and suggests that automatic image analysis of MN is a highly sensitive method for evaluating the effect of c-PAHs. Expand
Interlaboratory validation of a CD71‐based flow cytometric method (Microflow®) for the scoring of micronucleated reticulocytes in mouse peripheral blood
TLDR
The overall performance of mouse peripheral blood micronucleus tests is enhanced by the use of the flow cytometric scoring procedure, which offered advantages relative to microscopy‐based scoring, including a greater number of cells analyzed, much faster analysis times, and a greater degree of objectivity. Expand
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