Rapid detection of multidrug-resistant Mycobacterium tuberculosis by use of real-time PCR and high-resolution melt analysis.

@article{Ramirez2010RapidDO,
  title={Rapid detection of multidrug-resistant Mycobacterium tuberculosis by use of real-time PCR and high-resolution melt analysis.},
  author={Melissa V. Ramirez and Kelley C. Cowart and Patricia J. Campbell and Glenn P Morlock and David Sikes and Jonas M Winchell and James E Posey},
  journal={Journal of clinical microbiology},
  year={2010},
  volume={48 11},
  pages={4003-9}
}
The current study describes the development of a unique real-time PCR assay for the detection of mutations conferring drug resistance in Mycobacterium tuberculosis. The rifampicin resistance determinant region (RRDR) of rpoB and specific regions of katG and the inhA promoter were targeted for the detection of rifampin (RIF) and isoniazid (INH) resistance, respectively. Additionally, this assay was multiplexed to discriminate Mycobacterium tuberculosis complex (MTC) strains from nontuberculous… CONTINUE READING
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