Rapid detection of methicillin-resistant staphylococci by multiplex PCR.

@article{Kearns1999RapidDO,
  title={Rapid detection of methicillin-resistant staphylococci by multiplex PCR.},
  author={Angela M. Kearns and P. R. Seiders and Jeremy Wheeler and Robert H. Freeman and Michael W. Steward},
  journal={The Journal of hospital infection},
  year={1999},
  volume={43 1},
  pages={
          33-7
        }
}
A multiplex PCR was developed to detect the coagulase gene (coa; pathognomic of Staphylococcus aureus) and the mecA gene (characteristically encoding for methicillin resistance in staphylococci) in a single, rapid test. Suitable primers for the gene targets and an internal, amplification control were incorporated into a multiplex PCR assay, which was then optimized on a capillary air thermal cycler to improve the turnaround time of the test to approximately 1.5 hours. The assay was evaluated… CONTINUE READING

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