Rapid detection of methicillin resistance in coagulase-negative staphylococci by a penicillin-binding protein 2a-specific latex agglutination test.

@article{Horstkotte2001RapidDO,
  title={Rapid detection of methicillin resistance in coagulase-negative staphylococci by a penicillin-binding protein 2a-specific latex agglutination test.},
  author={Matthias Ansver Horstkotte and Johannes K-M Knobloch and Holly Rohde and Dietrich Mack},
  journal={Journal of clinical microbiology},
  year={2001},
  volume={39 10},
  pages={3700-2}
}
The detection of PBP 2a by the MRSA-Screen latex agglutination test with 201 clinical coagulase-negative staphylococci had an initial sensitivity of 98% and a high degree of specificity for Staphylococcus epidermidis strains compared to PCR for mecA. Determination of oxacillin MICs evaluated according to the new breakpoint (0.5 microg/ml) of the National Committee for Clinical Laboratory Standards exhibited an extremely low specificity for this population.