Rapid detection of intestinal carriage of Klebsiella pneumoniae producing KPC carbapenemase during an outbreak.

@article{Giani2012RapidDO,
  title={Rapid detection of intestinal carriage of Klebsiella pneumoniae producing KPC carbapenemase during an outbreak.},
  author={Tommaso Giani and Carlo Tascini and Fabio Arena and Ilaria Ciullo and Viola Conte and Alessandro Leonildi and Francesco Menichetti and Gian Maria Rossolini},
  journal={The Journal of hospital infection},
  year={2012},
  volume={81 2},
  pages={119-22}
}
Two different approaches are described for rapid detection of intestinal carriage of Klebsiella pneumoniae producing KPC-type carbapenemase (KPC-KP), based on PCR amplification of DNA extracts from rectal swabs (K-PCR), and on direct plating of rectal swabs on to MacConkey agar with a meropenem disc and a meropenem plus 3-aminophenylboronic acid disc (direct KPC screening test, DKST). K-PCR and DKST were tested with a total of 101 samples from 65 patients, during an outbreak. Although less… CONTINUE READING