Rapid detection of human immunodeficiency virus type 1 subtype e infection by PCR.

@article{Chen2002RapidDO,
  title={Rapid detection of human immunodeficiency virus type 1 subtype e infection by PCR.},
  author={M Y Chen and W Wang and Ming-Cheng Lee and S J Twu and S Wu and C F Lee},
  journal={Journal of clinical microbiology},
  year={2002},
  volume={40 10},
  pages={3805-9}
}
The CRF01_AE (subtype E) strain of human immunodeficiency virus type 1 (HIV-1), originally reported in Thailand, spread rapidly to and showed prevalence in several countries in Southeast Asia, including Taiwan. This strain was also found in other regions of the world. Based on sequence analysis of the vpu gene, a nested PCR assay including an outer primer pair and a subtype E-specific inner primer pair was developed in this study for rapid detection of subtype E viruses. It was tested with 397… CONTINUE READING
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