Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay.

@article{Hosaka2009RapidDO,
  title={Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay.},
  author={Norimitsu Hosaka and Nicaise Ndembi and Azumi Ishizaki and Seiji Kageyama and Kei Numazaki and Hiroshi Ichimura},
  journal={Journal of virological methods},
  year={2009},
  volume={157 2},
  pages={195-9}
}
A rapid one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the pol-integrase gene was developed to detect human immunodeficiency virus type 1 (HIV-1) group M. This HIV-1 RT-LAMP assay is simple and rapid, and amplification can be completed within 35min under isothermal conditions at 60 degrees C. The 100% detection limit of HIV-1 RT-LAMP was determined using a standard strain (WHO HIV-1 [97/656]) in octuplicate and found to be 120 copies/ml. The RT… CONTINUE READING