Rapid detection of herpes simplex virus and varicella-zoster virus infections by real-time PCR.

@article{Weidmann2003RapidDO,
  title={Rapid detection of herpes simplex virus and varicella-zoster virus infections by real-time PCR.},
  author={Manfred Weidmann and Ursula Meyer-Koenig and Frank T. Hufert},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 4},
  pages={1565-8}
}
The herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2) and varicella-zoster virus (VZV) can cause life-threatening infections of the central nervous system and lead to severe infections in immunocompromised subjects and newborns. In these cases, rapid diagnosis is crucial. We developed three different real-time PCR assays based on TaqMan chemistry for the LightCycler instrument to detect HSV-1, HSV-2, and VZV. When the TaqMan assays were compared to our in-house nested PCR assays, the test… CONTINUE READING