Rapid detection of allelic losses in brain tumours using microsatellite repeat markers and high-performance liquid chromatography

@article{Chernova2003RapidDO,
  title={Rapid detection of allelic losses in brain tumours using microsatellite repeat markers and high-performance liquid chromatography},
  author={Olga B. Chernova and Gene H. Barnett and John K Cowell},
  journal={British Journal of Cancer},
  year={2003},
  volume={88},
  pages={1889 - 1893}
}
High-performance liquid chromatography (HPLC) is a recently introduced high-capacity automated method for detecting unknown mutations (denaturing HPLC) or for sizing DNA fragments under nondenaturing conditions. We have adapted the HPLC method for detection of loss of heterozygosity (LOH) and used glial tumours as a model to evaluate its sensitivity and specificity in comparison to conventional denaturing polyacrylamide gel electrophoresis. A total of 20 oligodendrogliomas (grades II and III… CONTINUE READING
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