Rapid detection of a chromosomally mediated penicillin resistance-associated ponA mutation in Neisseria gonorrhoeae using a real-time PCR assay.

@article{VernelPauillac2006RapidDO,
  title={Rapid detection of a chromosomally mediated penicillin resistance-associated ponA mutation in Neisseria gonorrhoeae using a real-time PCR assay.},
  author={Fr{\'e}d{\'e}rique Vernel-Pauillac and Virginie Falcot and David W Whiley and Fabrice M{\'e}rien},
  journal={FEMS microbiology letters},
  year={2006},
  volume={255 1},
  pages={66-74}
}
The recent emergence of a decreased susceptibility of Neisseria gonorrhoeae strains to penicillin in New Caledonia has lead clinicians to operate a change in the treatment strategy. In addition, this important health issue has emphasized the need for a rapid means of detecting penicillin resistance in N. gonorrhoeae in order to select an effective treatment and limit the spread of resistant strains. In recent years, the use of fluorescence resonance energy transfer on the LightCycler has proven… CONTINUE READING

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