Rapid detection of Shiga toxin-producing bacteria in feces by multiplex PCR with molecular beacons on the smart cycler.

@article{Blanger2002RapidDO,
  title={Rapid detection of Shiga toxin-producing bacteria in feces by multiplex PCR with molecular beacons on the smart cycler.},
  author={Simon D B{\'e}langer and Maurice Boissinot and Christian Menard and François J. Picard and Michel G. Bergeron},
  journal={Journal of clinical microbiology},
  year={2002},
  volume={40 4},
  pages={1436-40}
}
We have developed a rapid (1-h) real-time fluorescence-based PCR assay with the Smart Cycler thermal cycler (Cepheid, Sunnyvale, Calif.) for the detection of Shiga toxin-producing Escherichia coli (STEC), as well as other Shiga toxin-producing bacteria. Based on multiple-sequence alignments, we have designed two pairs of PCR primers that efficiently amplify all variants of the Shiga toxin genes stx(1) and stx(2), respectively. These primer pairs were combined for use in a multiplex assay. Two… CONTINUE READING
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