Rapid detection of Salmonella from poultry by real-time polymerase chain reaction with fluorescent hybridization probes.

Abstract

Detection of Salmonella by bacteriologic methods is known to be time consuming. Therefore, we have developed a real-time probe-specific polymerase chain reaction (PCR) to rapidly detect Salmonella invA gene-based PCR products from chicken feces and carcasses by a fluorescence resonance energy transfer assay. The sensitivity and the specificity of this… (More)

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