Rapid detection of Oenococcus oeni in wine by real-time quantitative PCR.

@article{Pinzani2004RapidDO,
  title={Rapid detection of Oenococcus oeni in wine by real-time quantitative PCR.},
  author={Pamela Pinzani and L Bonciani and Mario Pazzagli and C. James Orlando and Simona Guerrini and Lisa Granchi},
  journal={Letters in applied microbiology},
  year={2004},
  volume={38 2},
  pages={118-24}
}
AIMS To develop a real-time polymerase chain reaction (PCR) method for rapid detection and quantification of Oenococcus oeni in wine samples for monitoring malolactic fermentation. METHODS AND RESULTS Specific primers and fluorogenic probe targeted to the gene encoding the malolactic enzyme of O. oeni were developed and used in real-time PCR assays in order to quantify genomic DNA either from bacterial pure cultures or wine samples. Conventional CFU countings were also performed. The PCR… CONTINUE READING

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