Rapid detection of JAK2 V617F mutation using high-resolution melting analysis with LightScanner platform.

@article{Qian2010RapidDO,
  title={Rapid detection of JAK2 V617F mutation using high-resolution melting analysis with LightScanner platform.},
  author={Jun Qian and Jiang Lin and Dong-ming Yao and Qin Chen and Gao-fei Xiao and Run-bi Ji and Yun Li and Jing Yang and Zhen Qian},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={2010},
  volume={411 23-24},
  pages={2097-100}
}
BACKGROUND Detection of the JAK2 mutation has recently been included under the essential diagnostic criteria for myeloproliferative neoplasm (MPN). High-resolution melt (HRM) curve analysis, a nongel-based, automated system, is introduced as a means of mutation scanning without the requirement of any post-PCR handling. METHODS We studied the sensitivity and reproducibility of LightScanner™ platform in the detection of JAK2 V617F mutation and the availability for diagnostic use in MPN… CONTINUE READING

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