Rapid detection and quantification of tyrosine decarboxylase gene (tdc) and its expression in gram-positive bacteria associated with fermented foods using PCR-based methods.

@article{Torriani2008RapidDA,
  title={Rapid detection and quantification of tyrosine decarboxylase gene (tdc) and its expression in gram-positive bacteria associated with fermented foods using PCR-based methods.},
  author={Sandra Torriani and Veronica Gatto and Silvia Sembeni and Rosanna Tofalo and Giovanna Suzzi and Nicoletta Belletti and Fausto Gardini and Sara Bover-Cid},
  journal={Journal of food protection},
  year={2008},
  volume={71 1},
  pages={93-101}
}
In this study, PCR-based procedures were developed to detect the occurrence and quantify the expression of the tyrosine decarboxylase gene (tdc) in gram-positive bacteria associated with fermented foods. Consensus primers were used in conventional and reverse transcription PCR to analyze a collection of 87 pure cultures of lactic acid bacteria and staphylococci. All enterococci, Staphylococcus epidermidis, Lactobacillus brevis, Lactobacillus curvatus, and Lactobacillus fermentum strains and 1… CONTINUE READING

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