Rapid detection and isolation of known and putative alpha-L-arabinofuranosidase genes using degenerate PCR primers.

@article{Park2007RapidDA,
  title={Rapid detection and isolation of known and putative alpha-L-arabinofuranosidase genes using degenerate PCR primers.},
  author={Jung-Mi Park and Nam Han and Tae-Jip Kim},
  journal={Journal of microbiology and biotechnology},
  year={2007},
  volume={17 3},
  pages={481-9}
}
alpha-L-Arabinofuranosidases (AFases; EC 3.2.1.55) are exo-type enzymes, which hydrolyze terminal nonreducing arabinose residues from various polysaccharides such as arabinan and arabinoxylan. Genome-wide BLAST search showed that various bacterial strains possess the putative AFase genes with well-conserved motif sequences at the nucleotide and amino acid sequence levels. In this study, two sets of degenerate PCR primers were designed and tested to detect putative AFase genes, based on their… CONTINUE READING