Corpus ID: 28511737

Rapid detection and identification of dengue viruses by polymerase chain reaction (PCR).

  title={Rapid detection and identification of dengue viruses by polymerase chain reaction (PCR).},
  author={Pa-thai Yenchitsomanus and P Sricharoen and I Jaruthasana and Sa-nga Pattanakitsakul and Sorachai Nitayaphan and Juthathip Mongkolsapaya and Prida Malasit},
  journal={The Southeast Asian journal of tropical medicine and public health},
  volume={27 2},
A polymerase chain reaction (PCR) method using sets of newly designed primers for rapid detection and simultaneous identification of dengue virus serotypes was developed and tested. The test is based on two sets of primers specific within the envelope (E) and non-structural (NS1) regions of the dengue-virus genome. Two sets of universal primers that bind to two target sequences which are shared by all the four serotypes of the virus within the E and NS1 regions are used. The resulting products… Expand
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