Rapid detection and differentiation of human pathogenic orthopox viruses by a fluorescence resonance energy transfer real-time PCR assay.

@article{Panning2004RapidDA,
  title={Rapid detection and differentiation of human pathogenic orthopox viruses by a fluorescence resonance energy transfer real-time PCR assay.},
  author={Marcus Panning and Marcel Asper and Stefanie Kramme and Herbert W. Schmitz and Christian Drosten},
  journal={Clinical chemistry},
  year={2004},
  volume={50 4},
  pages={
          702-8
        }
}
BACKGROUND The orthopox viruses that are pathogenic for humans include variola major virus (VAR), monkeypox virus (MPV), cowpox virus (CPV), and to a lesser extent, camelpox virus (CML) and vaccinia virus (VAC). PCR is a powerful tool to detect and differentiate orthopox viruses, and real-time PCR has the further advantages of rapid turnaround time, low risk of contamination, capability of strain differentiation, and use of multiplexed probes. METHODS We used real-time PCR with fluorescence… CONTINUE READING

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