Rapid demonstration of juxtaglomerular granules in mammals and birds.

@article{Smith1966RapidDO,
  title={Rapid demonstration of juxtaglomerular granules in mammals and birds.},
  author={C. Smith},
  journal={Stain technology},
  year={1966},
  volume={41 5},
  pages={
          291-4
        }
}
  • C. Smith
  • Published 1 September 1966
  • Chemistry, Medicine
  • Stain technology
Slices of kidney, 3 mm thick, are fixed in 10% formalin containing 5% K2Cr2O7 and 2.5% acetic acid (Smith's fixative) for 18 hr, washed 8 hr in running water, and 6 μ paraffin sections prepared as usual. The sections are brought to water, oxidized 1 min in a 1% KMnO4:1% H2SO4, mixture, decolorized with 1% Na2S2O5, stained 1 min in Ziehl's carbol-fuchsin and decolorized until pink in 35% ethanol containing 0.5% HCI. After 2 min washing, the slide with its adherent water is laid flat on a rack… Expand
Rapid demonstration of juxtaglomerular granules with alcoholic crystal violet.
  • K. Harada
  • Chemistry, Medicine
  • Stain technology
  • 1970
TLDR
Crystal violet, ethyl violet, new fuchsin, and victoria blue 4R were also found to be effective stains for the granules, and a prestaining oxidation with acidified KMnO4 was optional for other fixatives. Expand
Staining juxtaglomerular granules with basic fluorescent stains.
  • K. Harada
  • Chemistry, Medicine
  • Stain technology
  • 1969
TLDR
The stain is applied to paraffin sections of tissues fixed in 2% calcium acetate-10% formalin or in phosphate-buffered 10% formal in to produce characteristic colors in ultraviolet light. Expand
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TLDR
The present findings show that W-PAM can identify Ren-1 renin, but not Ren-2, rat or human renin. Expand
Comparative studies of the juxtaglomerular apparatus.
TLDR
The renin–angiotensin system (RAS) appeared first in primitive forms during the early evolution of bony fishes, and a variety of possible physiological roles of the RAS have been proposed. Expand
Morphologic observations on the summer cell of stilling in the interrenal gland of the american bullfrog (Rana catesbeiana)
  • T. Volk
  • Biology, Medicine
  • Zeitschrift für Zellforschung und Mikroskopische Anatomie
  • 2004
TLDR
The Stilling cell may secrete a renin-like substance that may function in an aldosterone-stimulating reninangiotensin system in the interrenal gland of the American bullfrog. Expand
Some effects of dihydroergocristine and of phentolamine mesylate on renal function in rats
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Single injections of dihydroergocristine (dhe) did not depress the systemic arterial pressure but antagonized the pressor effects of (‐)‐noradrenaline (100 μg, s.c.) in unanaesthetized rats, and hyperduric adrenaline (250 μg twice daily for 1 week) lowered the stores of growth hormone in the adenohypophysis. Expand
MORPHOLOGICAL CHANGES IN JUXTAGLOMERULAR CELLS BY LONG-TERM TREATMENT WITH AN ANGIOTENSIN-CONVERTING ENZYME INHIBITOR IN BEIGE RATS (CHEDIAK-HIGASHI SYNDROME)
TLDR
It is demonstrated that chronic ACE inhibition accelerates synthesis of JG granules including renin and inhibits secretion of renin, but it is not confirmed whether renin synthesis and secretion are affected by PAG II. Expand
Visualization of mitochondria and some other cytoplasmic structures in hematoxylin-eosin stained sections by fluorescence microscopy.
In hematoxylin (H)-eosin (E) stained, paraffin sections prepared after fixation in 10% neutral buffered formalin, fluorescence microscopy demonstrated mitochondria, juxtaglomerular granules and someExpand
Juxtaglomerular granules in subcellular fractions of guinea-pig kidney
TLDR
It is concluded, that the concept of adult guinea-pig kidney as an agranular model for the investigation of production and release of renin has to be modified. Expand
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  • Chemistry, Medicine
  • Stain technology
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A review of four methods for staining juxtaglomerular cells revealed that one method may be highly selective for juxtaglomerular granules (JGG) whereas another may stain general cytological featuresExpand
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