Rapid completion of mitosis and cytokinesis in PtK cells following release from nocodazole arrest.

Abstract

Mitotic PtK1 cells were arrested at nuclear envelope breakdown with the rapidly reversible microtubule inhibitor nocodazole. Addition of 1 microgram/ml nocodazole for 20 min resulted in complete blockage of spindle microtubule assembly but permitted other mitotic events to proceed such as chromosome condensation and cell rounding, typical of a c-mitosis… (More)

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