Rapid combined assay for Salmonella detection in food samples.

@article{Gad2000RapidCA,
  title={Rapid combined assay for Salmonella detection in food samples.},
  author={Istv{\'a}n Gad{\'o} and Peter F. Major and Margit Kir{\'a}ly and M G Pl{\'a}veczky},
  journal={Acta microbiologica et immunologica Hungarica},
  year={2000},
  volume={47 4},
  pages={445-56}
}
A rapid method was developed to detect salmonellae in food samples. The method gave a possibility to obtain results after 28 h 30 min. The preenrichment in buffered peptone water lasted for 6 h, the enrichment in Rappaport-Vassiliadis medium was applied for 18 h followed by PCR with INVA1-INVA2 primer pair, adapting Chiu and Ou's method. This procedure was suitable to demonstrate salmonella contamination at min. 10 cfu/25 g sample. Out of 18 samples there was a good agreement between the… CONTINUE READING