Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements

Abstract

To expand the repertoire of Cas9s available for genome targeting, we present a new in vitro method for the simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements. The method relies on the in vitro cleavage of plasmid libraries containing a randomized PAM as a function of Cas9-guide RNA complex concentration. Using this… (More)
DOI: 10.1186/s13059-015-0818-7

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@inproceedings{Karvelis2015RapidCO, title={Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements}, author={Tautvydas Karvelis and Giedrius Gasiūnas and Joshua Young and Greta Bigelyte and Arūnas Silanskas and Mark Cigan and Virginijus Siksnys}, booktitle={Genome Biology}, year={2015} }