Rapid biochemical tests for characterization of the Mycoplasmatales.

  title={Rapid biochemical tests for characterization of the Mycoplasmatales.},
  author={Janet M. Bradbury},
  journal={Journal of clinical microbiology},
  volume={5 5},
Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days.