Rapid assembly of the bacteriophage T4 core replication complex on a linear primer/template construct.

@article{Kaboord1993RapidAO,
  title={Rapid assembly of the bacteriophage T4 core replication complex on a linear primer/template construct.},
  author={Barbara Fenn Kaboord and Stephen J Benkovic},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 22},
  pages={10881-5}
}
DNA synthesis on a primed DNA substrate by bacteriophage T4 requires the assembly of a core replication complex consisting of the T4 DNA polymerase, a single-stranded binding protein (32 protein), and the accessory proteins 44/62 and 45. In this paper, we demonstrate the successful assembly of this core complex on a short linear primer/template system at levels of accessory proteins equivalent to the concentration of primer 3' ends. The key to this assembly is the presence of streptavidin… CONTINUE READING

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