Rapid assay of binding of tumor-promoting phorbol esters to protein kinase C1.

@article{Tanaka1986RapidAO,
  title={Rapid assay of binding of tumor-promoting phorbol esters to protein kinase C1.},
  author={Yukie Tanaka and Ruriko Miyake and Ushio Kikkawa and Yasutomi Nishizuka},
  journal={Journal of biochemistry},
  year={1986},
  volume={99 1},
  pages={257-61}
}
Protein kinase C is generally accepted to be a receptor protein of tumor-promoting phorbol esters. The binding of [3H]phorbol-12,13-dibutyrate to protein kinase C can be assayed by a rapid filtration procedure using a glass-fiber filter that has been treated with a cationic polymer, polyethylenimine. The phorbol ester specifically binds to the protein kinase only in the presence of phosphatidylserine and calcium. Non-specific binding is less than 10%, at most, of the total binding. The binding… CONTINUE READING

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