Rapid assay for catechol-O-methyltransferase activity by high-performance liquid chromatography-fluorescence detection.

@article{Hirano2005RapidAF,
  title={Rapid assay for catechol-O-methyltransferase activity by high-performance liquid chromatography-fluorescence detection.},
  author={Yoko Hirano and Makoto Tsunoda and Takashi Funatsu and Kazuhiro Imai},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2005},
  volume={819 1},
  pages={41-6}
}
A rapid assay for measuring the activities of catechol-O-methyltransferase (COMT) is described. The method is based on high-performance liquid chromatography (HPLC)-fluorescence detection, and includes on-line extraction of catecholamines with a precolumn, separation of norepinephrine (NE) and normetanephrine (NMN) on an ODS column, electrochemical… CONTINUE READING