Rapid and specific identification of medium-chain-length polyhydroxyalkanoate synthase gene by polymerase chain reaction

@article{Solaiman2000RapidAS,
  title={Rapid and specific identification of medium-chain-length polyhydroxyalkanoate synthase gene by polymerase chain reaction},
  author={Daniel K. Y. Solaiman and Richard D. Ashby and Thomas A. Foglia},
  journal={Applied Microbiology and Biotechnology},
  year={2000},
  volume={53},
  pages={690-694}
}
A polymerase chain reaction (PCR) protocol was developed for the specific detection of genes coding for type II polyhydroxyalkanoate (PHA) synthases. The primer-pair, I-179L and I-179R, was based on the highly conserved sequences found in the coding regions of Pseudomonas phaC1 and phaC2 genes. Purified genomic DNA or lysate of colony suspension can serve equally well as the target sample for the PCR, thus affording a simple and rapid screening of phaC1/C2-containing microorganisms. Positive… CONTINUE READING

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Production of novel polyhydroxyalkanoates by Pseudomonas stutzeri 1317 from glucose and soybean oil

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  • FEMSMicrobiol Lett
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