Rapid and specific detection of the thermostable direct hemolysin gene in Vibrio parahaemolyticus by loop-mediated isothermal amplification.

@article{Nemoto2009RapidAS,
  title={Rapid and specific detection of the thermostable direct hemolysin gene in Vibrio parahaemolyticus by loop-mediated isothermal amplification.},
  author={Jiro Nemoto and Chiyo Sugawara and Kenji Akahane and Keiji Hashimoto and Tadashi Kojima and Masanari Ikedo and Hirotaka Konuma and Yukiko Hara-Kudo},
  journal={Journal of food protection},
  year={2009},
  volume={72 4},
  pages={748-54}
}
Several investigators have reported that thermostable direct hemolysin (TDH) and TDH-related hemolysin are important virulence factors of Vibrio parahaemolyticus, but it has been difficult to detect these factors rapidly in seafood and other environmental samples. A novel nucleic acid amplification method, termed the loop-mediated isothermal amplification (LAMP), which amplifies DNA with high specificity and rapidity under isothermal conditions, was applied. In this study, we designed tdh gene… CONTINUE READING