Rapid and simultaneous determination of mycophenolic acid and its glucuronide conjugate in human plasma by ion-pair reversed-phase high-performance liquid chromatography using isocratic elution.

@article{Hosotsubo2001RapidAS,
  title={Rapid and simultaneous determination of mycophenolic acid and its glucuronide conjugate in human plasma by ion-pair reversed-phase high-performance liquid chromatography using isocratic elution.},
  author={Hideo Hosotsubo and Shirou Takahara and Yukito Kokado and Sompol Permpongkosol and Ji Yao Wang and T Tanaka and Kiyomi Matsumiya and Masaya Kitamura and Atsushi Okuyama and Hironori Sugimoto},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={2001},
  volume={753 2},
  pages={
          315-20
        }
}
A high-performance liquid chromatographic method has been developed for the simultaneous determination of mycophenolic acid (MPA) and its glucuronide conjugate (MPAG) in human plasma. The method involves protein precipitation with acetonitrile, followed by ion-pair reversed-phase chromatography on C18 column, with a 40 mM tetrabutyl ammonium bromide (TBA)-acetonitrile (65:35, v/v) mobile phase. A 20-microl volume of clear supernatant was injected after centrifugation, and the eluent was… CONTINUE READING

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