Rapid and simple ribozymic aminoacylation using three conserved nucleotides.

@article{Chumachenko2009RapidAS,
  title={Rapid and simple ribozymic aminoacylation using three conserved nucleotides.},
  author={Nataliya V Chumachenko and Yehor Novikov and Michael Yarus},
  journal={Journal of the American Chemical Society},
  year={2009},
  volume={131 14},
  pages={5257-63}
}
Selection-amplification finds new RNA enzymes (ribozymes) among randomized RNAs with flanking unvaried sequences (primer complements). Precise removal of 3'-primer before reaction selected aminoacylation from PheAMP in three cycles, yielding active RNAs (k(cat) = 12-20 min(-1)) using only three conserved nucleotides, acting independently of divalent ions. This unusually simple RNA active site encouraged study of the reaction via molecular mechanics-based free energy minimization. On this basis… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.

References

Publications referenced by this paper.

Enzyme Structure and Mechanism ; Freeman : New York , 1985

A. Fersht
J . AM . CHEM . SOC .

Similar Papers

Loading similar papers…